解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Constitutive activation of Wnt signaling plays an important role in colorectal and liver tumorigenesis. Cell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molec...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26394
更新日期:2017-12-01 00:00:00
abstract::Effective enzyme-mediated viscosity reduction, disaggregation, or "liquefaction," is required to overcome the rheological challenges resulting from the fibrous, hygroscopic nature of lignocellulosic biomass, particularly at the high solids loadings that will be required for an economically viable process. However, the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26381
更新日期:2017-11-01 00:00:00
abstract::Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26386
更新日期:2017-11-01 00:00:00
abstract::Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26353
更新日期:2017-10-01 00:00:00
abstract::Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26362
更新日期:2017-10-01 00:00:00
abstract::The paper reviews the state-of-the-art in computational modeling of thrombus formation and growth and related phenomena including platelet margination, activation, adhesion, and embolization. Presently, there is a high degree of empiricism in the modeling of thrombus formation. Based on the experimentally observed phy...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.26343
更新日期:2017-10-01 00:00:00
abstract::Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26330
更新日期:2017-09-01 00:00:00
abstract::The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26328
更新日期:2017-09-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells in culture are known to consume large amounts of nutrients and divert most of them toward byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. Glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yieldi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26313
更新日期:2017-08-01 00:00:00
abstract::Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26309
更新日期:2017-08-01 00:00:00
abstract::We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26270
更新日期:2017-08-01 00:00:00
abstract::A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N-acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycopr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26291
更新日期:2017-08-01 00:00:00
abstract::A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26249
更新日期:2017-07-01 00:00:00
abstract::The budding yeast Saccharomyces cerevisiae is equipped with G protein-coupled receptors (GPCR). Because the yeast GPCR signaling mechanism is partly similar to that of the mammalian system, S. cerevisiae can be used for a host of mammalian GPCR expression and ligand-mediated activation assays. However, currently avail...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26255
更新日期:2017-06-01 00:00:00
abstract::Nucleotide sugar-dependent ("Leloir") glycosyltransferases (GTs), represent a new paradigm for the application of biocatalytic glycosylations to the production of fine chemicals. However, it remains to be shown that GT processes meet the high efficiency targets of industrial biotransformations. We demonstrate in this ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26204
更新日期:2017-04-01 00:00:00
abstract::The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26205
更新日期:2017-04-01 00:00:00
abstract::The ability to capture cell-free DNA from the gastrointestinal tract, in a minimally invasive manner, could enhance our ability to diagnose gastrointestinal disease, or gain a better understanding of the spatial mapping of the intestinal microbiota. We, therefore, sought to identify a class of capture agents that coul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26207
更新日期:2017-04-01 00:00:00
abstract::The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26092
更新日期:2017-03-01 00:00:00
abstract::High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26185
更新日期:2017-03-01 00:00:00
abstract::A mechanistic model-based soft sensor is developed and validated for 550L filamentous fungus fermentations operated at Novozymes A/S. The soft sensor is comprised of a parameter estimation block based on a stoichiometric balance, coupled to a dynamic process model. The on-line parameter estimation block models the cha...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26187
更新日期:2017-03-01 00:00:00
abstract::Continuous manufacturing is currently being seriously considered in the biopharmaceutical industry as the possible new paradigm for producing therapeutic proteins, due to production cost and product quality related benefits. In this study, a monoclonal antibody producing CHO cell line was cultured in perfusion mode an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26069
更新日期:2017-02-01 00:00:00
abstract::There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.26060
更新日期:2017-01-01 00:00:00
abstract::Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26063
更新日期:2017-01-01 00:00:00
abstract::Cell morphology is an important macroscopic indicator of cellular physiology and is increasingly used as a mean of probing culture state in vitro. Phase contrast microscopy (PCM) is a valuable tool for observing live cells morphology over long periods of time with minimal culture artifact. Two general approaches are c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26064
更新日期:2017-01-01 00:00:00
abstract::Human skin-derived precursor cells (hSKPs) are multipotent adult stem cells found in the dermis of human skin. Incorporation of hSKPs into split-thickness skin grafts (STSGs), the current gold standard to treat severe burns or tissue resections, has been proposed as a treatment option to enhance skin wound healing and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26040
更新日期:2016-12-01 00:00:00
abstract::Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26026
更新日期:2016-12-01 00:00:00
abstract::Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26029
更新日期:2016-12-01 00:00:00
abstract::This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26030
更新日期:2016-12-01 00:00:00
abstract::Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26012
更新日期:2016-11-01 00:00:00
abstract::Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26009
更新日期:2016-11-01 00:00:00
abstract::Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selection, which limits targeted integration of multiple genes at multiple...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26002
更新日期:2016-11-01 00:00:00
abstract::Disulfide-rich peptides isolated from cone snails are of great interest as drug leads due to their high specificity and potency toward therapeutically relevant ion channels and receptors. They commonly contain the inhibitor cystine knot (ICK) motif comprising three disulfide bonds forming a knotted core. Here we repor...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25993
更新日期:2016-10-01 00:00:00
abstract::The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25976
更新日期:2016-10-01 00:00:00
abstract::A high-rate anaerobic digestion of food waste leachate were tested using intermittent continuously stirred tank reactors (iCSTRs) to evaluate how severe ammonia inhibition could be mitigated with internal dilution strategy, and to identify how bacterial and archaeal community respond in genus and species level. Experi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25968
更新日期:2016-09-01 00:00:00
abstract::Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25950
更新日期:2016-09-01 00:00:00
abstract::For all bacteria, nearly every antimicrobial fails since a subpopulation of the bacteria enter a dormant state known as persistence, in which the antimicrobials are rendered ineffective due to the lack of metabolism. This tolerance to antibiotics makes microbial infections the leading cause of death worldwide and make...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25963
更新日期:2016-09-01 00:00:00
abstract::Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25948
更新日期:2016-08-01 00:00:00
abstract::In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25946
更新日期:2016-08-01 00:00:00
abstract::Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25914
更新日期:2016-07-01 00:00:00
abstract::Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.25895
更新日期:2016-07-01 00:00:00